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仔豬日糧添加蛋白酶對(duì)豆粕致敏蛋白降解和腸道健康的影響
發(fā)布單位:天津瑞孚農(nóng)牧科技集團(tuán)有限公司
查看次數(shù)：10715
時(shí)間：2020-12-08
開(kāi)展兩個(gè)試驗(yàn)研究豆粕型基礎(chǔ)日糧中添加蛋白酶對(duì)豆粕致敏蛋白（大豆球蛋白和β-伴大豆球蛋白）降解和斷奶仔豬腸道健康的影響。
試驗(yàn)1中收集了兩個(gè)不同來(lái)源的兩個(gè)豆粕樣品，進(jìn)行豬胃體外降解實(shí)驗(yàn)，研究蛋白酶（15000 U/kg飼料原料）對(duì)豆粕致敏蛋白降解的影響。試驗(yàn)二，選用48頭斷奶仔豬（體重6.66kg），每批24頭，共兩批。豬只單獨(dú)飼喂在代謝籠中，總共4種日糧（12頭/日糧）。日糧為玉米-豆粕型，試驗(yàn)處理為2（豆粕1、豆粕2）×2（蛋白酶添加量為0、15000U/kg）雙因子試驗(yàn)。日糧飼喂10天，然后屠宰，檢測(cè)小腸組織形態(tài)、小腸滲透性，以及血清促炎因子水平。
豆粕1、豆粕2的粗蛋白含量分別為46.9%、47.7%，大豆球蛋白為14.0%和14.6%，β-伴大豆球蛋白為9.90%和10.3%。試驗(yàn)中未發(fā)現(xiàn)蛋白酶和豆粕之間有互作。蛋白酶的添加有提高大豆球蛋白體外胃液降解的趨勢(shì)（P=0.069）。日糧中添加蛋白酶降低了空腸異硫氰酸熒光素葡聚糖 4000 Da（腸道通透性的標(biāo)記探針，P=0.099），并顯著降低了血清TNF-α濃度（P=0.037）。蛋白酶對(duì)小腸組織形態(tài)沒(méi)有顯著影響。
總之，蛋白酶有提高大豆球蛋白胃液降解的趨勢(shì)，并降低腸道通透性、血清促炎因子的濃度，結(jié)果表明斷奶仔豬的豆粕型日糧中可以添加蛋白酶改善腸道健康。
Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets
Two experiments were conducted to determine the effects of protease supplementation on degradation of soybean meal (SBM) allergenic proteins (glycinin and β-conglycinin) and gut health of weaned pigs fed soybean meal-based diets. In experiment 1, 2 SBM samples from 2 different sources were subjected to porcine in vitro gastric degradation to determine the effects of protease (at 15,000 U/kg of feedstuff) on degradation of the soybean allergenic proteins. In experiment 2, 48 weaned pigs (body weight = 6.66 kg) were obtained in 2 batches of 24 pigs each. Pigs were individually housed in metabolic crates and fed 4 diets (12 pigs/diet). The diets were corn-based diet with SBM 1 or SBM 2 without or with protease at 15,000 U/kg of diet in 2 × 2 factorial arrangement. Diets were fed for 10 d and pigs were sacrificed on day 10 for measurement of small intestinal histomorphology, permeability of small intestine mounted in Ussing chambers, and serum concentration of pro-inflammatory cytokines. Two SBM sources (SBM 1 and SBM 2) contained 46.9% or 47.7% CP, 14.0% or 14.6% glycinin, and 9.90% or 10.3% β-conglycinin, respectively. Protease and SBM source did not interact on any of the response criteria measured in the current study. Protease supplementation tended to increase (P = 0.069) the in vitro gastric degradation of glycinin. Protease supplementation tended to reduce (P = 0.099) fluorescein isothiocyanate dextran 4,000 Da (which is a marker probe for intestinal permeability) flow in jejunum, and reduced (P = 0.037) serum TNF-α concentration. Protease did not affect small intestinal histomorphology. In conclusion, protease tended to increase gastric degradation of glycinin and reduce gut permeability, and serum concentration of pro-inflammatory cytokines, indicating that the protease used in the current study can be added to SBM-based diets for weanling pigs to improve gut health.
文章來(lái)源：豬營(yíng)養(yǎng)國(guó)際論壇
【免責(zé)聲明】：文章來(lái)源于網(wǎng)絡(luò)，我們對(duì)文中陳述觀點(diǎn)判斷保持中立，并不對(duì)文章觀點(diǎn)負(fù)責(zé)。僅供讀者參考。版權(quán)屬于原作者。
試驗(yàn)1中收集了兩個(gè)不同來(lái)源的兩個(gè)豆粕樣品，進(jìn)行豬胃體外降解實(shí)驗(yàn)，研究蛋白酶（15000 U/kg飼料原料）對(duì)豆粕致敏蛋白降解的影響。試驗(yàn)二，選用48頭斷奶仔豬（體重6.66kg），每批24頭，共兩批。豬只單獨(dú)飼喂在代謝籠中，總共4種日糧（12頭/日糧）。日糧為玉米-豆粕型，試驗(yàn)處理為2（豆粕1、豆粕2）×2（蛋白酶添加量為0、15000U/kg）雙因子試驗(yàn)。日糧飼喂10天，然后屠宰，檢測(cè)小腸組織形態(tài)、小腸滲透性，以及血清促炎因子水平。
豆粕1、豆粕2的粗蛋白含量分別為46.9%、47.7%，大豆球蛋白為14.0%和14.6%，β-伴大豆球蛋白為9.90%和10.3%。試驗(yàn)中未發(fā)現(xiàn)蛋白酶和豆粕之間有互作。蛋白酶的添加有提高大豆球蛋白體外胃液降解的趨勢(shì)（P=0.069）。日糧中添加蛋白酶降低了空腸異硫氰酸熒光素葡聚糖 4000 Da（腸道通透性的標(biāo)記探針，P=0.099），并顯著降低了血清TNF-α濃度（P=0.037）。蛋白酶對(duì)小腸組織形態(tài)沒(méi)有顯著影響。
總之，蛋白酶有提高大豆球蛋白胃液降解的趨勢(shì)，并降低腸道通透性、血清促炎因子的濃度，結(jié)果表明斷奶仔豬的豆粕型日糧中可以添加蛋白酶改善腸道健康。
Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets
Two experiments were conducted to determine the effects of protease supplementation on degradation of soybean meal (SBM) allergenic proteins (glycinin and β-conglycinin) and gut health of weaned pigs fed soybean meal-based diets. In experiment 1, 2 SBM samples from 2 different sources were subjected to porcine in vitro gastric degradation to determine the effects of protease (at 15,000 U/kg of feedstuff) on degradation of the soybean allergenic proteins. In experiment 2, 48 weaned pigs (body weight = 6.66 kg) were obtained in 2 batches of 24 pigs each. Pigs were individually housed in metabolic crates and fed 4 diets (12 pigs/diet). The diets were corn-based diet with SBM 1 or SBM 2 without or with protease at 15,000 U/kg of diet in 2 × 2 factorial arrangement. Diets were fed for 10 d and pigs were sacrificed on day 10 for measurement of small intestinal histomorphology, permeability of small intestine mounted in Ussing chambers, and serum concentration of pro-inflammatory cytokines. Two SBM sources (SBM 1 and SBM 2) contained 46.9% or 47.7% CP, 14.0% or 14.6% glycinin, and 9.90% or 10.3% β-conglycinin, respectively. Protease and SBM source did not interact on any of the response criteria measured in the current study. Protease supplementation tended to increase (P = 0.069) the in vitro gastric degradation of glycinin. Protease supplementation tended to reduce (P = 0.099) fluorescein isothiocyanate dextran 4,000 Da (which is a marker probe for intestinal permeability) flow in jejunum, and reduced (P = 0.037) serum TNF-α concentration. Protease did not affect small intestinal histomorphology. In conclusion, protease tended to increase gastric degradation of glycinin and reduce gut permeability, and serum concentration of pro-inflammatory cytokines, indicating that the protease used in the current study can be added to SBM-based diets for weanling pigs to improve gut health.
文章來(lái)源：豬營(yíng)養(yǎng)國(guó)際論壇
【免責(zé)聲明】：文章來(lái)源于網(wǎng)絡(luò)，我們對(duì)文中陳述觀點(diǎn)判斷保持中立，并不對(duì)文章觀點(diǎn)負(fù)責(zé)。僅供讀者參考。版權(quán)屬于原作者。
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